How to Reconstitute Peptides with Bacteriostatic Water (For Research Use Only)

Disclaimer: This information is provided strictly for laboratory research purposes. These products are not intended for human consumption, medical use, or veterinary applications.

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What is Bacteriostatic Water?

Bacteriostatic water is sterile water containing 0.9% benzyl alcohol, which helps prevent bacterial growth. Researchers use it to dissolve lyophilized (freeze-dried) peptides because it supports sterility and stability in controlled lab settings.

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Why Reconstitute Peptides?

Peptides are usually supplied as a powder to maintain stability during storage. Before they can be used in experiments, they need to be reconstituted (dissolved) into a liquid solution. Bacteriostatic water helps maintain sterility during this process.

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Materials Needed

• Lyophilized peptide vial

• Bacteriostatic water (sterile, laboratory grade)

• Sterile syringe and needle

• Alcohol swabs

• Laboratory gloves and safety equipment

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Step-by-Step Guide

Step 1: Prepare the Work Area
Work in a clean, sterile environment. Wipe down the area with disinfectant and wear gloves.

Step 2: Clean the Vial Tops
Wipe the rubber stoppers of both vials (peptide and bacteriostatic water) with alcohol swabs.

Step 3: Draw Bacteriostatic Water
Use a sterile syringe to draw the desired amount of bacteriostatic water. The volume depends on the concentration needed for your research.

Step 4: Add Water to the Peptide Vial
Inject the water slowly against the side of the vial instead of directly onto the powder to avoid foaming.

Step 5: Gently Mix
Swirl the vial gently until the powder dissolves completely. Do not shake vigorously.

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Storage After Reconstitution

• Store reconstituted peptides in a refrigerator (2–8°C).

• Avoid repeated freeze-thaw cycles.

• Follow your institution’s approved protocols for storage and handling.

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Final Notes

Reconstituting peptides with bacteriostatic water is a common lab practice that supports consistency, sterility, and accuracy in research settings.

For research use only. Not for human consumption.